Journal: Nucleic Acids Research

Article Title: RDML: structured language and reporting guidelines for real-time quantitative PCR data

doi: 10.1093/nar/gkp056

Figure Lengend Snippet: Real-time PCR data exchange with RDML. RDML acts as a facilitator of, and common language for exchange of qPCR data and related information between users, software programs and databases.

Article Snippet: The RDML project is supported by companies providing real-time PCR instruments and reagents (Bio-Rad Laboratories, Roche, Applied Biosystems) or reagents only (Eurogentec, Primer Design), developing PCR data analysis software (Biogazelle, LabonNet and MultiD Analysis) and organizing qPCR courses (Biogazelle, TATAA Biocenter), as well as a number of opinion leaders, core facility managers and researchers active in the field of qPCR, including the developers of geNorm, qBase, RTprimerDB and REST.

Techniques: Real-time Polymerase Chain Reaction, Software

Journal: Biophysical Reviews

Article Title: Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening

doi: 10.1007/s12551-025-01280-3

Figure Lengend Snippet: Proposed hit screening procedure using DSF followed by ITC/MST methods with main advantages of these approaches and marked further steps for drug design

Article Snippet: Of course, this is always incorporated in commercial software (ITC Data Analysis in Origin ; TA Instruments NanoAnalyze Software Getting Started Guide ) and should be in homemade analysis procedures (SokołOwska et al. ).

Techniques:

Journal: Biophysical Reviews

Article Title: Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening

doi: 10.1007/s12551-025-01280-3

Figure Lengend Snippet: Comparison of most common biophysical method for quantifying ligand binding

Article Snippet: Of course, this is always incorporated in commercial software (ITC Data Analysis in Origin ; TA Instruments NanoAnalyze Software Getting Started Guide ) and should be in homemade analysis procedures (SokołOwska et al. ).

Techniques: Comparison, Binding Assay, High Throughput Screening Assay, Differential Scanning Calorimetry, SPR Assay, Nuclear Magnetic Resonance, Labeling, Mass Spectrometry, Isothermal Titration Calorimetry, Microscale Thermophoresis, Fluorescence, Spectroscopy, Förster Resonance Energy Transfer

Journal: Biophysical Reviews

Article Title: Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening

doi: 10.1007/s12551-025-01280-3

Figure Lengend Snippet: Isothermal titration calorimetry. Standard binding experiment with cartoon representing the bound and unbound protein and two dominating heat effects caused by ligand binding and mixing of components. The heat of each injection gradually reduces as free protein decreases upon ligand binding

Article Snippet: Of course, this is always incorporated in commercial software (ITC Data Analysis in Origin ; TA Instruments NanoAnalyze Software Getting Started Guide ) and should be in homemade analysis procedures (SokołOwska et al. ).

Techniques: Isothermal Titration Calorimetry, Binding Assay, Ligand Binding Assay, Injection

Journal: Biophysical Reviews

Article Title: Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening

doi: 10.1007/s12551-025-01280-3

Figure Lengend Snippet: Comparison of ITC data fitted separately (left panel) and globally (right panel). The example shown is the binding of 4,7-dibromo-5,6-diiodo-1H-benzotriazole to hCK2α, results adapted from (Paprocki et al. ). Black circles show experimental data, grey ones indicate data removed from the analysis, thick blue lines represent the fitted model of two sequential binding sites, and red ones represent the fitted model of a single binding site. The dotted lines boarder the 95% confidence limits for a particular model

Article Snippet: Of course, this is always incorporated in commercial software (ITC Data Analysis in Origin ; TA Instruments NanoAnalyze Software Getting Started Guide ) and should be in homemade analysis procedures (SokołOwska et al. ).

Techniques: Comparison, Binding Assay

Journal: Biophysical Reviews

Article Title: Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening

doi: 10.1007/s12551-025-01280-3

Figure Lengend Snippet: List of studies, in which all three methods: DSF, MST, and ITC were used

Article Snippet: Of course, this is always incorporated in commercial software (ITC Data Analysis in Origin ; TA Instruments NanoAnalyze Software Getting Started Guide ) and should be in homemade analysis procedures (SokołOwska et al. ).

Techniques: Binding Assay, Titration, Kinase Assay